Floofty is nonbinary and uses they/them pronouns.

Available on: Epic Games Store, Steam, Playstation 4, Playstation 5, Nintendo Switch, Xbox One, Xbox Series X/S

Frisk, Chara, Napstablook, and Monster Kid are all referred to with they/them pronouns.

In the Nintendo Switch version, after you defeat Mad Dummy, its ghost goes on to possess a Mew Mew doll, becoming Mad Mew Mew.

Available on: PC, Steam, Playstation 4, Playstation Vita, Nintendo Switch, Xbox One

** I am aware of the debates regarding Naoto, but I still choose to include him for personal reasons I will not change my mind about. Do not attempt to debate me because you will just waste time for us both. You are welcome to disagree and make your own list if this inclusion bothers you.

Naoto is initially introduced as a man and presents as such, wearing the male school uniform and purposefully deepening his voice. When you get to his dungeon, his Shadow reveals that he was not assigned male at birth, and is actually a woman who disguised himself as a man to avoid misogyny in his line of work, going as far as to desire sex reassignment surgery. His dungeon also features a lot of medical themes.

The scene that takes place the next day when Naoto returns to school and joins the party features NPCs gossiping about Naoto's gender, similarly to an outing of a trans person. Naoto is shown very uncomfortable with this.

After this reveal, Naoto continues to bind their chest and wear more masculine clothing, but should the player want to unlock Naoto's romance route, the protagonist has to tell Naoto to be more feminine. During an intimate moment between the protagonist and Naoto where he expresses his wish to have been born a boy, you are able to initiate the romance route by responding, "I'm glad you're a girl."
Later, after confessing your love, you are able to ask him to change the sound of his voice in a higher pitch to sound more feminine. If you do so, Naoto comes to school in a girl's uniform during the Christmas event, despite being very visibly uncomfortable and quickly asking to change back into his masculine clothes.

Naoto also expresses discomfort in a hot springs scene where his naked body is paired alongside his cis female companions.

Shigenori Soejima, the art and character designer of Persona, says that Naoto was intended to be male all along, but that would have made for a boring story that lacks the conflict of the canon story.

An official Line Sticker also shows that Naoto binds his chest with bandages.

Also take into consideration that all of Naoto's Personas are male mythical figures.

Available on: Playstation Vita, Steam, Microsoft Store, Playstation 4, Playstation 5 (through backwards compatability), Nintendo Switch, Xbox One, Xbox Series X/S

Mama's gender in her character file is listed as gender-neutral.

Available on: Steam, Playstation 4, Xbox One, Nintendo Switch

The game does not make the player confirm their gender. All hairstyles, clothes, and makeup are usable, regardless of whether the player chooses a masculine or feminine style for their character.

Available on: Nintendo Switch

The waitress Erica is a trans woman. At one point she begins a sexual relationship with a man who has a crush on her, Toby, which he later expresses regret about in the True Ending due to finding out her assigned birth gender.

Available on: Playstation 3, Xbox 360, Steam

Flea, the game's antagonist, is gender neutral and at one point proclaims "Male or female? What difference does it make? I've got the power and power is beautiful."

Available on: SNES, PlayStation, Nintendo DS, iOS, Android, Steam

** I am aware of the debates regarding Chihiro, but I still choose to include her for personal reasons I will not change my mind about. Do not attempt to debate me because you will just waste time for us both. You are welcome to disagree and make your own list if this inclusion bothers you.

Chihiro is initially introduced as a female student and presents as such.

In Chapter 2, Monokuma threatens to reveal a "secret" that each of the individual characters have. Because of this, Chihiro attempts to build trust by telling her secret to Mondo, a person she admires, which is that she was born male and dresses up as a girl due to bullying she faced as a child for "not being manly enough" and "being so weak for a boy". Chihiro then asks Mondo to help her become stronger, but this only lead to Mondo being overcome by rage and jealousy at Chihiro’s strength and courage, feeling humiliated and "unmanly", and as a result, he strikes Chihiro over the head with a dumbbell, killing her.

Chihiro's assigned gender is outed to the other characters during the following trial.

Available on: Playstation Portable, Android, iOS, Playstation Vita, Steam, Playstation 4, Nintendo Switch, Xbox One

Kris is referred to with they/them pronouns.

Available on: Steam, PC, Nintendo Switch

Kris is referred to with they/them pronouns.

Available on: Steam, PC, Nintendo Switch

Krem Aclassi is a trans man. Krem tells the Inquisitor to let him know if they need help binding and players are able to ask questions about his transition. ("Why pass as a man?" / "Are you a woman?" / "And the rest of your crew?" / "When did you know?")

Available on: Steam, EA Store, Playstation 3, Playstation 4, Xbox 360, Xbox One

Gogo uses the masculine pronoun "ore" in the Japanese version, and they cannot equip female-exclusive equipment, but their canonical gender is unknown. Both the Japanese and English scripts describe Gogo's gender as difficult to tell.

Available on: SNES, Playstation 1, Game Boy Advance, Steam, iOS, Android, Playstation 4, Nintendo Switch

Other characters, in-game bios, and official books refer to Mangle with conflicting gendered pronouns and terms. In this game, Phone Guy refers to Mangle as male, but in the custom night "Ladies Night," Mangle is included with Toy Chica and Withered Chica.
In 2015, Scott Cawthon even addressed the question of Mangle's gender by (albeit jokingly) stating "Is Mangle a boy or a girl? The answer is yes."

The Puppet is also referred to with either they/them or it/its.

Available on: Steam, Android, iOS, Nintendo Switch, PlayStation 4, Xbox One

Funtime Foxy's gender is ambiguous, being referred to with either he/him or she/her. The Custom Night descriptions use he/him pronouns, but similarly to Mangle, F. Foxy is also included in the custom night "Girls' Night."

Available on: Steam, Android, iOS, Nintendo Switch, PlayStation 4, Xbox One

Mizuki is heavily implied to be nonbinary/transfeminine. Their gender is labelled as "?" on the official website for the game. They were male-presenting in middle school, wearing the male school uniform and having shorter hair. In-game, some characters such as An and Miku refer to Mizuki with "they/them" pronouns, although the English translation avoids using pronouns at all. In the Japanese version, the pronoun "boku" is used, which typically refers to young boys casually.

Available on: iOS, Android

Felix states that The Oracle does not have a physical form or a gender.
(Other characters are also stated or hinted to be trans in episodes 2 and 3)

Available on: Steam, Itch.io

It's hinted that Charlotte is a trans girl.
A memory titled "Feminine" depicts a younger Charlotte talking with Aiden, who encourages her to be more feminine by wearing dresses and growing her hair out.

Available on: Steam, Itch.io

Charles is genderfluid as stated in his character profile.

Available on: Steam, Itch.io

Janeva's gender is not stated, though they assert that they are "not one of [Aloy's] sisters" and are clearly a soldier for Carja, a profession exclusively for men.

Available on: Steam, Playstation 4

See Mass Effect (2007).

Available on: Xbox 360, Playstation 3, Microsoft Store, EA Store, Steam, Playstation 4 (Legendary Edition, remaster, also on Steam and Xbox One)

See Mass Effect (2007).

Availabe on: Xbox 360, Playstation 3, Wii U, Microsoft store, Steam, Playstation 4 (Legendary Edition, remaster, also on Steam and Xbox One)

See Mass Effect (2007).

Available on: Xbox One, Microsoft Store, Steam, Playstation 4, Playstation 5 (through backwards compatibility)

In 2011, the villain Sweet Tooth was introduced in the Super Moshi mission Candy Catastrophe. Sweet Tooth's gender is never specified, and they are referred to either with they/them or no pronouns at all.

Part of their character bio in the book Moshi Monsters Character Encyclopedia reads: "Nobody knows if Sweet Tooth is a he or a she, and it's best not to ask, as the last monster who did ended up in moshpital!"

(Formerly) available on: PC

Bea's friend Jackie is a trans woman.
Scott Benson clarified on CuriousCat that he had intended for this to come up at some point in the first version of the game, but the information had gotten lost in the shuffle as scenes were rewritten and trimmed, and he didn't realize it was still missing from the final cut until it was too late. As of Weird Autumn edition, Bea now mentions to Mae that the name of Jackie's single-person band was "Trans World Order Give Them Hell"

The symbol on Mae's shirt is also a common nonbinary symbol, though Scott Benson was unaware of this and had not intended for Mae to be nonbinary.

Available on: Itch.io, PC, Steam, Playstation 4, Xbox One, Nintendo Switch, iOS

** I am aware of the debates regarding Naoto, but I still choose to include him for personal reasons I will not change my mind about. Do not attempt to debate me because you will just waste time for us both. You are welcome to disagree and make your own list if this inclusion bothers you.

Naoto is initially introduced as a man and presents as such, wearing the male school uniform and purposefully deepening his voice. When you get to his dungeon, his Shadow reveals that he was not assigned male at birth, and is actually a woman who disguised himself as a man to avoid misogyny in his line of work, going as far as to desire sex reassignment surgery. His dungeon also features a lot of medical themes.

The scene that takes place the next day when Naoto returns to school and joins the party features NPCs gossiping about Naoto's gender, similarly to an outing of a trans person. Naoto is shown very uncomfortable with this.

After this reveal, Naoto continues to bind their chest and wear more masculine clothing, but should the player want to unlock Naoto's romance route, the protagonist has to tell Naoto to be more feminine. During an intimate moment between the protagonist and Naoto where he expresses his wish to have been born a boy, you are able to initiate the romance route by responding, "I'm glad you're a girl."
Later, after confessing your love, you are able to ask him to change the sound of his voice in a higher pitch to sound more feminine. If you do so, Naoto comes to school in a girl's uniform during the Christmas event, despite being very visibly uncomfortable and quickly asking to change back into his masculine clothes.

Naoto also expresses discomfort in a hot springs scene where his naked body is paired alongside his cis female companions.

Shigenori Soejima, the art and character designer of Persona, says that Naoto was intended to be male all along, but that would have made for a boring story that lacks the conflict of the canon story.

An official Line Sticker also shows that Naoto binds his chest with bandages.

Also take into consideration that all of Naoto's Personas are male mythical figures.

Available on: Playstation 2

See Persona 4 (2008) or Persona 4 Golden (2012).

Available on: Arcade, Playstation 3, Xbox 360, Xbox One, Xbox Series X/S (through backwards compatability)

See Persona 4 (2008) or Persona 4 Golden (2012).

Available on: Aracade, Playstation 3, Xbox 360, Playstation 4, Nintendo Switch, Steam

Naoto expresses discomfort when dressed in feminine alternate costumes.

See Persona 4 (2008) or Persona 4 Golden (2012) for more info.

Available on: Playstation Vita, Playstation 4 (Endless Night Collection)

See Persona 4 (2008) or Persona 4 Golden (2012).

Available on: Nintendo 3DS

Naoto's gender is revealed similarly to an outing of a trans person.

See Persona 4 (2008) or Persona 4 Golden (2012) for more info.

Available on: Nintendo 3DS

Nova is a Pokémon Trainer found at the Battle Maison. She is a Beauty, an exclusively female trainer class, who states that she used to be a Black Belt, an exclusively male trainer class. In the Japanese version of the game, she explicitly credits her transformation to medical science, but this was cut from the English version.

Available on: Nintendo 3DS

The protagonist Flower Kid is intended to represent all players and is never gendered, and Tim-Tam is exclusively referred to with they/them pronouns.

The developers also support Dr. Habit being interpreted as a trans man.

Available on: Steam, Nintendo Switch, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

Michel was assigned female at birth and raised accordingly, but experienced gender dysphoria from an early age. While recovering from an illness, his shoulders broadened and voice deepened, making him appear masculine. After that, his backstory consists almost entirely of him being imprisoned and misgendered by his family, not only for his changed appearance, but for his insistence that he is a man and always has been.

** This is an exception to my "no intersex characters" rule, as Michel is both a transgender man and intersex.

Available on: Steam, iOS, Playstation Vita, Playstation 4, Nintendo Switch, Nintendo 3DS (exclusive to Japan)

Lev is a trans boy. He had been kicked out of his religious community known as the Seraphites for shaving his head and identifying as a man. During a confrontation with some Seraphites, they angrily refer to Lev by his deadname.

Available on: Playstation 4

Players are able to add top surgery scars, binders, tucking underwear, and shapewear to their Sims, along with being able to select their Sims' pronouns (either selecting he/him, she/her, they/them, or entering custom ones).

Available on: PC, Steam, Xbox One, Playstation 4

The city councilwoman of San Francisco, Miranda Comay, is a trans woman. She is a longtime friend of the protagonist Marcus and his family, and helps Dedsec in their mission to take down the central Operating System and the corrupt company behind it.
At one point in the game, she mentions that the religious group Church of the New Dawn hacked her medical records and released footage of her sex reassignment surgery to the public.

Available on: Steam, Ubisoft Store, Epic Games Store, Playstation 4, Xbox One

Venus is a trans girl whose pronouns in the narration change from "he" to "she."

Available on: Itch.io, Steam

TOMCAT uses they/them pronouns and Sympathy is a bearded person that uses she/her. Melody Flores and Nina James are both trans women. Turing is usually gendered male by humans because of their blue casing and their namesake, but has no real preference on gender themselves. You are also able to choose your pronouns when creating a character by selecting from a pre-determined list or by entering your own.

Available on: Playstation 4, Playstation Vita, Nintendo Switch, Xbox One, iOS, Android, Steam

The English translation features a sidequest from a "transfixing woman" to retreive her "blue pill bottle", which she says contains hormones that are very important to her.

Available on: Playstation Portable

An NPC will greet the party with "Mr, Ms, Mx Murakumo!", implying that at least one member of the player-created party could be nonbinary.

Available on: Playstation Portable

Ryker is nonbinary.

Available on: Itch.io

The main character is nonbinary.

Available on: Steam, Itch.io

The player character can be nonbinary and you can select he/him, she/her, they/them, or enter custom pronouns for them.

Available on: Steam, Itch.io

The protagonist Ceyda is a trans woman.

Available on: Steam, Itch.io, Humble Store

Kea is nonbinary.

Available on: Steam, Itch.io

Lyall is nonbinary.

Available on: Steam

Mama appears again in this game, and an exchange with her suggests that she had gotten bottom surgery.

Uchikoshi has also confirmed that Marco is nonbinary. He explains that in the Japanese version, any pronouns are not used to refer to Marco, but since it is difficult to construct sentences without pronouns in English and because players might be confused* if "they" or "ze" suddenly appeared in dialogue, the localization team decided to use he/him.

(* Uchikoshi clarifies that he does not mean confused as in misunderstanding the meaning of "they" or "ze" pronouns, but rather, "Now suppose your mind is filled with many thoughts about the case. And in that state, if any other important piece of information (unrelated to the case) jumps into your head, it will be a mix of both and messed up, right? That's what I meant by confused.")

Available on: Steam, Nintendo Switch, PlayStation 4, Xbox One

The protagonist's gender is unspecified.

Available on: Steam, Itch.io, Nintendo Switch, Playstation 4

Jo is nonbinary.

Available on: Android, iOS

The game is about investigating the discarded phone of a bisexual trans woman named Samantha.

Available on: anormallostphone.com, Itch.io, iOS, Android, Steam, Nintendo Switch

Bloodhound is nonbinary and Catalyst is a trans woman.

Available on: EA Store, Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S, Nintendo Switch

The game allows you to pick a masculine or feminine presenting MC, and you are able to choose either she/her, he/him, or they/them for pronouns regardless of which appearance you choose.

Available on: Steam, Itch.io, Android

The player character can be nonbinary.
If romanced, Ashley will confess to not feeling 100% boy or girl.

Available on: Steam, Nintendo Switch, Playstation 4, Xbox One

Rhapsody is nonbinary and the player character can also be nonbinary.

Available on: Steam, Itch.io, Nintendo Switch, Playstation 5, Xbox Series X/S

Quinn is nonbinary and the player character is able to be nonbinary. You can also change the gender of your character at any time.

Available on: Steam, Itch.io, Nintendow Switch, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

An NPC named Ned is a trans man.

Available on: Steam, Ubisoft Store, Playstation 4, Xbox One

The protagonist Haru is a trans woman.

Available on: Steam, Nintendo Switch, GOG.com, Itch.io, Xbox One, Xbox Series X/S, Playstation 4, Playstation 5

The Underling is genderless.

Available on: Steam, Itch.io, Android

Nocturne is a trans woman.

Regardless of which body type you select for your character, you are able to choose any voice that you'd like as well as what pronouns you'd like your character to be referred to with. You can also choose what set of genitalia you'd like your character to have.

Available on: PC, Steam, Playstation 5, Xbox Series X/S

Mizhena is a trans woman who explains to the main character that she picked her name after transitioning.

Available on: PC, Steam, iOS, Android, Nintendo Switch, Playstation 4, Xbox One

Guillo was crafted as a sexless puppet and has the voices of both a man and a woman simultaneously. Characters also refer to Guillo with it/its pronouns.

Available on: Gamecube, Nintendo Switch (Baten 1 & 2 remaster)

Mai Natsume is a trans woman. Originally being born a man, at one point she had became one with the Nameless Grimoire, which had transformed her into a woman in order for her to become its host. Though initially shocked and embarrassed, throughout her story she learns that she enjoys being a girl and looks at her change in a positive light. When given a chance to go back to her old body, she decides not to, as she identifies as a woman.

Available on: Arcade (exclusive to Japan), Steam, Playstation 3, Playstation 4, Nintendo Switch

Mai Natsume is a trans woman. Originally being born a man, at one point she had became one with the Nameless Grimoire, which had transformed her into a woman in order for her to become its host. Though initially shocked and embarrassed, throughout her story she learns that she enjoys being a girl and looks at her change in a positive light. When given a chance to go back to her old body, she decides not to, as she identifies as a woman.

Available on: Arcade, Steam, Nintendo Switch, Playstation 4, Xbox One, Xbox Series X/S

Gabriella (formerly introduced as a male character named Gabriel) is revealed to have transitioned when she was resurrected and summoned through a portal created by the game's Singularity Generator.

Available on: Steam

NakedToaster is nonbinary and uses he/they pronouns. The player character can also be nonbinary.

Available on: Itch.io

FL4K uses they/them pronouns and has a pin on the collar of their coat that had the number 2 in binary crossed out, as well as the nonbinary flag colors.

Available on: Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

Sophie is a trans woman.

Available on: Steam, Itch.io, Android, iOS

Sawyer, Holmes, and Rowan are all nonbinary.

Available on: Steam, Windows, Nintendo Switch, Xbox One, Xbox Series X/S, PlayStation 5

Fel and Reed are nonbinary and both use they/them pronouns.

Available on: Steam

Zariel is an angel who outright states in their bio "Mortal or angel, male or female – I am above such primitive distinctions."

Available on: iOS, Android, Steam, Epic Games Store, Ubisoft Connect, Nintendo Switch, PlayStation 4, Xbox One

Birdo, a transgender woman, is imprisoned for using the woman's bathroom, and asks the player to find proof of her being female so she could be set free.

Available on: Wii (exclusive to Japan)

Shirley, Cyrille, Rainbow, and Amie are nonbinary, Franklyn and Heen are trans (the latter specifically referred to as a "transcat"), and Clarkii has no specified gender.

Available on: iOS, Android

Ravi and High Priest Raziel are nonbinary.

Available on: Itch.io

The waitress Erica is a trans woman.

Available on: Playstation Vita (exclusive to Japan), Playstation 4, Nintendo Switch

Madeline is a trans woman and is shown to have a rainbow flag and trans pride flag in her room. Her identity was also later confirmed by developer Maddy Thorson.

Available on: Steam, Itch.io, Nintendo Switch, Playstation 4, Xbox One, Epic Games Store

Iochi is nonbinary.

Available on: Nintendo Switch, Android, iOS, Playstation Vita

Pizza is referred to with they/them and you can select the pronouns for your player character.

Available on: Steam, PlayStation 4, PlayStation 5, Nintendo Switch, Xbox One, Xbox Series X/S

Jiwon is genderfluid.

Available on: Steam

The protagonist can be nonbinary and the love interest Ilar is nonbinary.

Available on: Steam, Itch.io, Android

The game features a trans woman named Yasmin. Part of her bio in the game reads:
"Yasmin was Marid Audran's long-time girlfriend, although she hadn't been born female."

Available on: Microsoft DOS

The protagonist is agender and Sabine and Peake are nonbinary.

Available on: Steam, Nintendo Switch, Xbox One, Xbox Series X/S, PlayStation 4, PlayStation 5

Several characters are nonbinary/genderless and referred to with either they/them or it/its pronouns.

Available on: iOS, Android

Several characters are nonbinary/genderless and referred to with either they/them or it/its pronouns.

Available on: iOS, Android

Several characters are nonbinary/genderless and referred to with either they/them or it/its pronouns.

Available on: iOS, Android

Catbat is nonbinary and uses they/them pronouns.

Available on: Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

The protagonist can be nonbinary and the love interests' genders can either be selected or randomized, which both include a nonbinary option.

Available on: Steam, Android, iOS

Artemis is nonbinary.

Available on: Steam, Itch.io

Bolt is genderqueer and uses they/them pronouns.

Available on: Steam, PlayStation 4, PlayStation Vita, iOS, Android, Xbox One, Nintendo Switch

Lamb is genderless.

Available on: PC, Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S, Nintendo Switch

Billie is nonbinary.

Available on: Steam

The game's character creator will allow V to be transgender/nonbinary.

An NPC named Claire who gives you racing sidequests is also a trans woman, and the back of her truck features a trans flag decal.

Available on: Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

You can choose to be referred to with either he/him, she/her, they/them, or no pronouns at all.

Available on: Itch.io

The protagonist is gender neutral.

Available on: Itch.io

Tubarão, a trans man from Rainbow Six Siege, is featured in this as a playable character/legendary skin.

Available on: Steam, Windows, Playstation 4, Xbox One, Nintendo Switch, Android, iOS, Stadia, Xbox Series X/S, Playstation 5

Nimbus is nonbinary, Oryx is a trans man, and Micah is a trans woman.

Available on: Bungie.net, Steam, Playstation 4, Xbox One, Playstation 5, Xbox Series X/S

Jester uses they/them pronouns and Robot uses it/its pronouns, the latter used to be human and voluntarily became a machine, its former pronouns never being revealed.

Available on: Steam, Itch.io, Nintendo Switch, Xbox One, Xbox Series X/S, Android, iOS, Playstation 4, Playstation 5

Dating Scammer Suguru states that they are nonbinary ("Knowing my name and looking at me, you'd probably guess I'm a guy, right? Well, you're wrong! I'm neither a guy nor a girl!").

Available on: Steam, Playstation Vita, Playstation 4, Nintendo Switch

Mindy Blanchard is a trans woman.

Available on: Steam, Windows, Playstation 4, Xbox One

A visual novel where you play as a trans woman who is worried about whether she passes or not. With the help of a magical webpage, she becomes a ghost and peeks into the minds of others to determine how they feel about her.

Available on: Itch.io

Ben and Peter are trans men, Breadley (he/him) is genderfluid, Yello, Soup, Moss (all they/them), and Dr. Mystery (any pronouns) are nonbinary, and Mago (he/him, sometimes "she" or "it") is agender.

Available on: Itch.io