Frisk, Chara, Napstablook, and Monster Kid are all referred to with they/them pronouns.

In the Nintendo Switch version, after you defeat Mad Dummy, its ghost goes on to possess a Mew Mew doll, becoming Mad Mew Mew.

Available on: PC, Steam, Playstation 4, Playstation Vita, Nintendo Switch, Xbox One

Chaos is referred to with they/them.

Available on: Steam, Windows, Mac, Nintendo Switch, PlayStation 4, PlayStation 5, Xbox One, Xbox Series X/S, iOS

The Knight and their siblings are genderless.

Available on: PC, Steam, Nintendo Switch, Playstation 4, Xbox One

Madeline is a trans woman and is shown to have a rainbow flag and trans pride flag in her room. Her identity was also later confirmed by developer Maddy Thorson.

Available on: Steam, Itch.io, Nintendo Switch, Playstation 4, Xbox One, Epic Games Store

The game does not make the player confirm their gender. All hairstyles, clothes, and makeup are usable, regardless of whether the player chooses a masculine or feminine style for their character.

Available on: Nintendo Switch

The game's character creator will allow V to be transgender/nonbinary.

An NPC named Claire who gives you racing sidequests is also a trans woman, and the back of her truck features a trans flag decal.

Available on: Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

Lev is a trans boy. He had been kicked out of his religious community known as the Seraphites for shaving his head and identifying as a man. During a confrontation with some Seraphites, they angrily refer to Lev by his deadname.

Available on: Playstation 4

Janeva's gender is not stated, though they assert that they are "not one of [Aloy's] sisters" and are clearly a soldier for Carja, a profession exclusively for men.

Available on: Steam, Playstation 4

Bloodhound is nonbinary and Catalyst is a trans woman.

Available on: EA Store, Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S, Nintendo Switch

The English version of the game and social media accounts avoid using gendered pronouns when referring to Blanche. (The Spanish, French, Italian, and Brazilian Portuguese translations refer to them as female. The German translations referred to them as female as well, but German social media accounts stopped using gendered pronouns for Blanche after 2020.)

Available on: Android, iOS

Venture is nonbinary and uses they/them pronouns.

Available on: Nintendo Switch, PlayStation 4, PlayStation 5, Windows, Xbox One, and Xbox Series X/S

** I am aware of the debates regarding Naoto, but I still choose to include him for personal reasons I will not change my mind about. Do not attempt to debate me because you will just waste time for us both. You are welcome to disagree and make your own list if this inclusion bothers you.

Naoto is initially introduced as a man and presents as such, wearing the male school uniform and purposefully deepening his voice. When you get to his dungeon, his Shadow reveals that he was not assigned male at birth, and is actually a woman who disguised himself as a man to avoid misogyny in his line of work, going as far as to desire sex reassignment surgery. His dungeon also features a lot of medical themes.

The scene that takes place the next day when Naoto returns to school and joins the party features NPCs gossiping about Naoto's gender, similarly to an outing of a trans person. Naoto is shown very uncomfortable with this.

After this reveal, Naoto continues to bind their chest and wear more masculine clothing, but should the player want to unlock Naoto's romance route, the protagonist has to tell Naoto to be more feminine. During an intimate moment between the protagonist and Naoto where he expresses his wish to have been born a boy, you are able to initiate the romance route by responding, "I'm glad you're a girl."
Later, after confessing your love, you are able to ask him to change the sound of his voice in a higher pitch to sound more feminine. If you do so, Naoto comes to school in a girl's uniform during the Christmas event, despite being very visibly uncomfortable and quickly asking to change back into his masculine clothes.

Naoto also expresses discomfort in a hot springs scene where his naked body is paired alongside his cis female companions.

Shigenori Soejima, the art and character designer of Persona, says that Naoto was intended to be male all along, but that would have made for a boring story that lacks the conflict of the canon story.

An official Line Sticker also shows that Naoto binds his chest with bandages.

Also take into consideration that all of Naoto's Personas are male mythical figures.

Available on: Playstation Vita, Steam, Microsoft Store, Playstation 4, Playstation 5 (through backwards compatability), Nintendo Switch, Xbox One, Xbox Series X/S

** I am aware of the debates regarding Chihiro, but I still choose to include her for personal reasons I will not change my mind about. Do not attempt to debate me because you will just waste time for us both. You are welcome to disagree and make your own list if this inclusion bothers you.

Chihiro is initially introduced as a female student and presents as such.

In Chapter 2, Monokuma threatens to reveal a "secret" that each of the individual characters have. Because of this, Chihiro attempts to build trust by telling her secret to Mondo, a person she admires, which is that she was born male and dresses up as a girl due to bullying she faced as a child for "not being manly enough" and "being so weak for a boy". Chihiro then asks Mondo to help her become stronger, but this only lead to Mondo being overcome by rage and jealousy at Chihiro’s strength and courage, feeling humiliated and "unmanly", and as a result, he strikes Chihiro over the head with a dumbbell, killing her.

Chihiro's assigned gender is outed to the other characters during the following trial.

Available on: Playstation Portable, Android, iOS, Playstation Vita, Steam, Playstation 4, Nintendo Switch, Xbox One

Giovanna's bestiary description reads as follows: "Giovanna resented being assigned Mage at birth, and proudly took up the way of the broom and pointed hat. Her sisters, who claim they want to 'protect witches' spaces,' have sworn to oppose her and all who fight by her side. Maybe one day they'll accept that anybody can be a Sassy Witch if they truly want to be."

Available on: Steam, Windows, Mac, Xbox One, Xbox Series X/S, Nintendo Switch, Android, IOS

Nova is a Pokémon Trainer found at the Battle Maison. She is a Beauty, an exclusively female trainer class, who states that she used to be a Black Belt, an exclusively male trainer class. In the Japanese version of the game, she explicitly credits her transformation to medical science, but this was cut from the English version.

Available on: Nintendo 3DS

Clove is nonbinary and uses they/them pronouns.

Available on: Windows

Liara T'Soni belongs to a single gendered race called the Asari, a species that has traditionally feminine traits but reproduces by means other than sexual reproduction. Liara can also state that "male" and "female" have no real meaning to Asari when female Shepard asks if she would like to enter a relationship despite them both being women.

Available on: Xbox 360, Playstation 3, Steam, Microsoft Store, EA Store, Playstation 4 (Legendary Edition, remaster, also on Steam and Xbox One)

Players are able to add top surgery scars, binders, tucking underwear, and shapewear to their Sims, along with being able to select their Sims' pronouns (either selecting he/him, she/her, they/them, or entering custom ones).

Available on: PC, Steam, Xbox One, Playstation 4

All members of the Hearthian species are nonbinary and use they/them pronouns.

Available on: Playstation 4, Playstation 5, Steam, Xbox One, Xbox Series X/S, Nintendo Switch, Epic Games Store

Kris is referred to with they/them pronouns.

Available on: Steam, PC, Nintendo Switch

Nocturne is a trans woman.

Regardless of which body type you select for your character, you are able to choose any voice that you'd like as well as what pronouns you'd like your character to be referred to with. You can also choose what set of genitalia you'd like your character to have.

Available on: PC, Steam, Playstation 5, Xbox Series X/S

Nimbus is nonbinary, Oryx is a trans man, and Micah is a trans woman.

Available on: Bungie.net, Steam, Playstation 4, Xbox One, Playstation 5, Xbox Series X/S

In Osa's Biography, her transition is mentioned. It was also confirmed by Ubisoft that she is transgender. Sens is also nonbinary and uses they/them pronouns, as seen in their Biography and Psychological Report.
Tubarão is also a trans man. (Something of note is that he is also a playable character/legendary skin in Dead By Daylight)


Availble on: Ubisoft Store, Epic Games Store, Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

See Mass Effect (2007).

Available on: Xbox 360, Playstation 3, Microsoft Store, EA Store, Steam, Playstation 4 (Legendary Edition, remaster, also on Steam and Xbox One)

Eden is nonbinary and uses they/them pronouns.

Available on: Steam, Playstation 4, Playstation Vita, Xbox One, New Nintendo 3DS, Wii U, iOS, Nintendo Switch, Playstation 5, Xbox Series X/S

Tubarão, a trans man from Rainbow Six Siege, is featured in this as a playable character/legendary skin.

Available on: Steam, Windows, Playstation 4, Xbox One, Nintendo Switch, Android, iOS, Stadia, Xbox Series X/S, Playstation 5

Nova is a Pokémon Trainer found at the Battle Maison. She is a Beauty, an exclusively female trainer class, who states that she used to be a Black Belt, an exclusively male trainer class. In the Japanese version of the game, she explicitly credits her transformation to medical science, but this was cut from the English version.

Available on: Nintendo 3DS

Flea, the game's antagonist, is gender neutral and at one point proclaims "Male or female? What difference does it make? I've got the power and power is beautiful."

Available on: SNES, PlayStation, Nintendo DS, iOS, Android, Steam

The city councilwoman of San Francisco, Miranda Comay, is a trans woman. She is a longtime friend of the protagonist Marcus and his family, and helps Dedsec in their mission to take down the central Operating System and the corrupt company behind it.
At one point in the game, she mentions that the religious group Church of the New Dawn hacked her medical records and released footage of her sex reassignment surgery to the public.

Available on: Steam, Ubisoft Store, Epic Games Store, Playstation 4, Xbox One

From Wikipedia:
In the first-edition manual for the North American release of the game, Birdo is referred to as a "male who believes that he is a female" and would rather be called "Birdetta", making her the first transgender character for Nintendo.
In the Japanese version, Birdo is referred as "Catherine" where it is also stated that the character is a man who wishes to be a woman. In later printings, mention of Birdo being male was omitted, and further not included in most later games with the character.

Available on: Arcade, FAMICOM, NES, SNES, Nintendo 3DS, Wii, Wii U, Nintendo Switch Online

Zariel is an angel who outright states in their bio "Mortal or angel, male or female – I am above such primitive distinctions."

Available on: iOS, Android, Steam, Epic Games Store, Ubisoft Connect, Nintendo Switch, PlayStation 4, Xbox One

FL4K uses they/them pronouns and has a pin on the collar of their coat that had the number 2 in binary crossed out, as well as the nonbinary flag colors.

Available on: Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

Mindy Blanchard is a trans woman.

Available on: Steam, Windows, Playstation 4, Xbox One

See Mass Effect (2007).

Availabe on: Xbox 360, Playstation 3, Wii U, Microsoft store, Steam, Playstation 4 (Legendary Edition, remaster, also on Steam and Xbox One)

Kris is referred to with they/them pronouns.

Available on: Steam, PC, Nintendo Switch

Ticker is a trans woman and Xaku is nonbinary.

Available on: Windows, Playstation 4, Xbox One, Nintendo Switch, PlayStation 5, Xbox Series X/S

Arcsys confirmed Testament was never intended to be male and always went by they/them pronouns.

Bridget's arcade mode story revolves around her being unsure whether she is truly happy as a boy and is being her true self, which is revealed to be her questioning her gender identity in one of the endings. Depending on how well the player performs, she makes progress towards figuring it out and accepting her new identity, with one of the endings directy showing her coming out to Goldlewis Dickinson and Ky Kiske, proudly stating that she is a girl.

Availble on: Arcade (exclusive to Japan), Playstation 4, Playstation 5, Steam, Xbox One, Xbox Series X

An NPC named Ned is a trans man.

Available on: Steam, Ubisoft Store, Playstation 4, Xbox One

Other characters, in-game bios, and official books refer to Mangle with conflicting gendered pronouns and terms. In this game, Phone Guy refers to Mangle as male, but in the custom night "Ladies Night," Mangle is included with Toy Chica and Withered Chica.
In 2015, Scott Cawthon even addressed the question of Mangle's gender by (albeit jokingly) stating "Is Mangle a boy or a girl? The answer is yes."

The Puppet is also referred to with either they/them or it/its.

Available on: Steam, Android, iOS, Nintendo Switch, PlayStation 4, Xbox One

Vivian is a trans woman in the Japanese version. Her elder sister Beldam unfortunately misgenders her and refers to her a cross-dresser, but this is not present in the English version.

Available on: Gamecube

One of the party members Quina belongs to a genderless race called the Qu and is referred to with "s/he" throughout the game.

Available on: Playstation, Playstation Network, Playstation 4, Nintendo Switch, Steam, Microsoft Store, Xbox One, Android, iOS

The faerie folk of the First are referred to with they/them pronouns.

Available on: PlayStation 5, PlayStation 4, Xbox Series X/S, Windows, Mac, Steam

Bea's friend Jackie is a trans woman.
Scott Benson clarified on CuriousCat that he had intended for this to come up at some point in the first version of the game, but the information had gotten lost in the shuffle as scenes were rewritten and trimmed, and he didn't realize it was still missing from the final cut until it was too late. As of Weird Autumn edition, Bea now mentions to Mae that the name of Jackie's single-person band was "Trans World Order Give Them Hell"

The symbol on Mae's shirt is also a common nonbinary symbol, though Scott Benson was unaware of this and had not intended for Mae to be nonbinary.

Available on: Itch.io, PC, Steam, Playstation 4, Xbox One, Nintendo Switch, iOS

Krem Aclassi is a trans man. Krem tells the Inquisitor to let him know if they need help binding and players are able to ask questions about his transition. ("Why pass as a man?" / "Are you a woman?" / "And the rest of your crew?" / "When did you know?")

Available on: Steam, EA Store, Playstation 3, Playstation 4, Xbox 360, Xbox One

Lamb is genderless.

Available on: PC, Steam, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S, Nintendo Switch

Gogo uses the masculine pronoun "ore" in the Japanese version, and they cannot equip female-exclusive equipment, but their canonical gender is unknown. Both the Japanese and English scripts describe Gogo's gender as difficult to tell.

Available on: SNES, Playstation 1, Game Boy Advance, Steam, iOS, Android, Playstation 4, Nintendo Switch

Birdo makes an appearance in this game.

Available on: Gameboy Advance, Wii U, Nintendo Switch Online

Instead of asking the player to pick a boy or girl inkling, the character creator simply gives players the option to pick their "style" and are referred to with they/them.

Available on: Nintendo Switch

Taylor is a disembodied brain in a jar and claims to not believe in gender. It requests to be referred to as either "it" or, if that makes someone uncomfortable, "they."

Available on: PC, Steam, Playstation Vita, Playstation 4, Nintendo Switch

Chromie is a trans woman and Pelagos is a trans man.

Available on: Windows, Mac

** I am aware of the debates regarding Naoto, but I still choose to include him for personal reasons I will not change my mind about. Do not attempt to debate me because you will just waste time for us both. You are welcome to disagree and make your own list if this inclusion bothers you.

Naoto is initially introduced as a man and presents as such, wearing the male school uniform and purposefully deepening his voice. When you get to his dungeon, his Shadow reveals that he was not assigned male at birth, and is actually a woman who disguised himself as a man to avoid misogyny in his line of work, going as far as to desire sex reassignment surgery. His dungeon also features a lot of medical themes.

The scene that takes place the next day when Naoto returns to school and joins the party features NPCs gossiping about Naoto's gender, similarly to an outing of a trans person. Naoto is shown very uncomfortable with this.

After this reveal, Naoto continues to bind their chest and wear more masculine clothing, but should the player want to unlock Naoto's romance route, the protagonist has to tell Naoto to be more feminine. During an intimate moment between the protagonist and Naoto where he expresses his wish to have been born a boy, you are able to initiate the romance route by responding, "I'm glad you're a girl."
Later, after confessing your love, you are able to ask him to change the sound of his voice in a higher pitch to sound more feminine. If you do so, Naoto comes to school in a girl's uniform during the Christmas event, despite being very visibly uncomfortable and quickly asking to change back into his masculine clothes.

Naoto also expresses discomfort in a hot springs scene where his naked body is paired alongside his cis female companions.

Shigenori Soejima, the art and character designer of Persona, says that Naoto was intended to be male all along, but that would have made for a boring story that lacks the conflict of the canon story.

An official Line Sticker also shows that Naoto binds his chest with bandages.

Also take into consideration that all of Naoto's Personas are male mythical figures.

Available on: Playstation 2

WX-78 is nonbinary and uses they/them pronouns.

Available on: Klei Game Store, Steam, Android, iOS, Playstation 3, Playstation 4, Playstation Vita, Xbox One, Wii U, Nintendo Switch

Juniper is nonbinary and referred to with they/them (or nongendered pronouns/conflicting gendered terms in other languages).

Available on: Nintendo Switch

You are able to choose he/him, she/her, or they/them pronouns for your player character.

Available on: Steam, Windows, XBOX Series X/S

Niko's gender is unspecified.

Available on: Steam, Nintendo Switch, Playstation 4, Xbox One

Mama's gender in her character file is listed as gender-neutral.

Available on: Steam, Playstation 4, Xbox One, Nintendo Switch

Bolt is genderqueer and uses they/them pronouns.

Available on: Steam, PlayStation 4, PlayStation Vita, iOS, Android, Xbox One, Nintendo Switch

See Mass Effect (2007).

Available on: Xbox One, Microsoft Store, Steam, Playstation 4, Playstation 5 (through backwards compatibility)

The waitress Erica is a trans woman. At one point she begins a sexual relationship with a man who has a crush on her, Toby, which he later expresses regret about in the True Ending due to finding out her assigned birth gender.

Available on: Playstation 3, Xbox 360, Steam

Limstella is genderless.

Available on: Game Boy Advance

From Wikipedia:
Poison is introduced in Final Fight (1989), where she is either a pre-op or post-op trans woman, depending on the region where the game was released.

She is an antagonistic NPC in the arcade beat 'em up games Final Fight (1989) and Mighty Final Fight (1993). She is a playable character in the fighting games Final Fight Revenge (1999), Street Fighter X Tekken (2012), Ultra Street Fighter IV (2014) & Street Fighter V: Arcade Edition (2018).

In Street Fighter V: Arcade Edition, her identity is alluded to more often. Certain dialogue during a conversation with Lucia alludes to the fact that she was born with a gender not matching with her inner being. One of Poison's win quotes, "Being gossiped about is proof of my popularity!" seemingly alludes to the controversy and ambiguity surrounding her gender identity.

Available on: Arcade, Playstation Network, Playstation 3, Playstation 4, Xbox Live Arcade, Steam

When the game initially released, Hainly Abrams would readily inform the player that she is transgender and reveal the name she was given at birth. She tells the player that she left the Milky Way galaxy searching for greater acceptance of her gender identity. The game was later patched so that she would only reveal this information if the player developed a supportive relationship with her.

Available on: EA Store, Steam, Playstation 4, Xbox One

The real life professional skater Leo Baker is featured in this game. He is transgender and nonbinary, using he/they pronouns.

Available on: Playstation 4, Playstation 5, Xbox One, Xbox Series X/S, Windows, Nintendo Switch

Floofty is nonbinary and uses they/them pronouns.

Available on: Epic Games Store, Steam, Playstation 4, Playstation 5, Nintendo Switch, Xbox One, Xbox Series X/S

The waitress Erica is a trans woman.

Available on: Playstation Vita (exclusive to Japan), Playstation 4, Nintendo Switch

Paolo is a trans man. He was once in the Fuerzas Nacionales de Defensa but was expelled after his father, a drill instructor, found out about his identity. According to Matías Alonso, Paolo underwent top surgery without any anesthetic and barely flinched.

Ubisoft themselves have also described Paolo with the following: "He's trans in a country that refuses to accept him for who he is."

Available on: Playstation 4, Playstation 5, Xbox One, Xbox Series X/S, Steam, PC

An argonian NPC named Chal-Maht is a trans man, stating he used to be born female before changing to a man through the Hist. Similarly, Kud-Nakal, a friend and love interest of Chal-Maht's, is a trans woman who also changed to female through the Hist.

Available on: PC, Playstation 4, Playstation 5, Xbox One, Xbox Series X/S

See Persona 4 (2008) or Persona 4 Golden (2012).

Available on: Aracade, Playstation 3, Xbox 360, Playstation 4, Nintendo Switch, Steam

Funtime Foxy's gender is ambiguous, being referred to with either he/him or she/her. The Custom Night descriptions use he/him pronouns, but similarly to Mangle, F. Foxy is also included in the custom night "Girls' Night."

Available on: Steam, Android, iOS, Nintendo Switch, PlayStation 4, Xbox One

While Luka is referred to as a boy by the other characters, as well as several official websites generally using he/him pronouns, Luka does mention several times that she wishes to have been born a girl, and that she wishes "the things you can see would match the things you, well, can't."

Available on: Steam, Xbox 360, PlayStation Portable, Android, iOS, PlayStation 3, PlayStation Vita, PlayStation 4

Naoto expresses discomfort when dressed in feminine alternate costumes.

See Persona 4 (2008) or Persona 4 Golden (2012) for more info.

Available on: Playstation Vita, Playstation 4 (Endless Night Collection)

Melting, Plant, Robot, Horror, Skeleton, and Frog are genderless/have no confirmed gender.

Available on: Steam, Playstation 4, Playstation Vita, Nintendo Switch, Xbox One

The game can randomly generate characters that mention them transitioning in their backstory.

Available on: Steam, Windows, Mac, Linux, Playstation 4, Xbox One

Jester uses they/them pronouns and Robot uses it/its pronouns, the latter used to be human and voluntarily became a machine, its former pronouns never being revealed.

Available on: Steam, Itch.io, Nintendo Switch, Xbox One, Xbox Series X/S, Android, iOS, Playstation 4, Playstation 5

Mai Natsume is a trans woman. Originally being born a man, at one point she had became one with the Nameless Grimoire, which had transformed her into a woman in order for her to become its host. Though initially shocked and embarrassed, throughout her story she learns that she enjoys being a girl and looks at her change in a positive light. When given a chance to go back to her old body, she decides not to, as she identifies as a woman.

Available on: Arcade, Steam, Nintendo Switch, Playstation 4, Xbox One, Xbox Series X/S

The Daycare Attendant is referred to with it/its, and they/them when they become Eclipse.

Available on: PlayStation 4, PlayStation 5, Windows, Stadia, Xbox One, Xbox Series X/S, Nintendo Switch

Mama appears again in this game, and an exchange with her suggests that she had gotten bottom surgery.

Uchikoshi has also confirmed that Marco is nonbinary. He explains that in the Japanese version, any pronouns are not used to refer to Marco, but since it is difficult to construct sentences without pronouns in English and because players might be confused* if "they" or "ze" suddenly appeared in dialogue, the localization team decided to use he/him.

(* Uchikoshi clarifies that he does not mean confused as in misunderstanding the meaning of "they" or "ze" pronouns, but rather, "Now suppose your mind is filled with many thoughts about the case. And in that state, if any other important piece of information (unrelated to the case) jumps into your head, it will be a mix of both and messed up, right? That's what I meant by confused.")

Available on: Steam, Nintendo Switch, PlayStation 4, Xbox One

The protagonist is agender and Sabine and Peake are nonbinary.

Available on: Steam, Nintendo Switch, Xbox One, Xbox Series X/S, PlayStation 4, PlayStation 5

Naoto's gender is revealed similarly to an outing of a trans person.

See Persona 4 (2008) or Persona 4 Golden (2012) for more info.

Available on: Nintendo 3DS

An aid-worker NPC named Sya is a trans woman who used magic to transition.

As of the latest DLC (released in 2022), a character named Yao has been added, who is nonbinary and uses they/them pronouns.

Available on: Windows, Steam

Mai Natsume is a trans woman. Originally being born a man, at one point she had became one with the Nameless Grimoire, which had transformed her into a woman in order for her to become its host. Though initially shocked and embarrassed, throughout her story she learns that she enjoys being a girl and looks at her change in a positive light. When given a chance to go back to her old body, she decides not to, as she identifies as a woman.

Available on: Arcade (exclusive to Japan), Steam, Playstation 3, Playstation 4, Nintendo Switch

Oz is nonbinary.

Available on: Steam, Nintendo Switch, PlayStation 4, Xbox One

Michel was assigned female at birth and raised accordingly, but experienced gender dysphoria from an early age. While recovering from an illness, his shoulders broadened and voice deepened, making him appear masculine. After that, his backstory consists almost entirely of him being imprisoned and misgendered by his family, not only for his changed appearance, but for his insistence that he is a man and always has been.

** This is an exception to my "no intersex characters" rule, as Michel is both a transgender man and intersex.

Available on: Steam, iOS, Playstation Vita, Playstation 4, Nintendo Switch, Nintendo 3DS (exclusive to Japan)

Enkidu is genderless.

Available on: Android, iOS

See Persona 4 (2008) or Persona 4 Golden (2012).

Available on: Arcade, Playstation 3, Xbox 360, Xbox One, Xbox Series X/S (through backwards compatability)

Alice is strongly trans coded, see Your Time To Shine: Island Existence (2019).

Available on: Steam, vgperson's page (browser)

Mangle, the Puppet, and Funtime Foxy make an appearance in this game.

Available on: Windows, Android, iOS, Nintendo Switch, PlayStation 4, Xbox One

From Wikipedia:
Poison is introduced in Final Fight (1989), where she is either a pre-op or post-op trans woman, depending on the region where the game was released.

She is an antagonistic NPC in the arcade beat 'em up games Final Fight (1989) and Mighty Final Fight (1993). She is a playable character in the fighting games Final Fight Revenge (1999), Street Fighter X Tekken (2012), Ultra Street Fighter IV (2014) & Street Fighter V: Arcade Edition (2018).

In Street Fighter V: Arcade Edition, her identity is alluded to more often. Certain dialogue during a conversation with Lucia alludes to the fact that she was born with a gender not matching with her inner being. One of Poison's win quotes, "Being gossiped about is proof of my popularity!" seemingly alludes to the controversy and ambiguity surrounding her gender identity.

Available on: Playstation 3, Xbox 360, iOS, Playstation Vita, Steam

Pizza is referred to with they/them and you can select the pronouns for your player character.

Available on: Steam, PlayStation 4, PlayStation 5, Nintendo Switch, Xbox One, Xbox Series X/S

Dating Scammer Suguru states that they are nonbinary ("Knowing my name and looking at me, you'd probably guess I'm a guy, right? Well, you're wrong! I'm neither a guy nor a girl!").

Available on: Steam, Playstation Vita, Playstation 4, Nintendo Switch

The protagonist Tyler is a trans man.

Available on: Steam, Xbox One

A substory involves an NPC named Ayaka, who is a trans woman. Talking to her at the Bantam bar will involve her asking for Kiryu's advice on how to ask out a man she is romantically interested in, eventually confessing to Kiryu about her birth assignment. She goes on into explaining her transition and how she underwent gender reassignment surgery, which is why she was unsure whether to ask out to the guy she likes since she worries about how he'll react. Kiryu is initially surprised by this, and you have the option of saying it's a bit difficult to come to terms with, but he is overall supportive of Ayaka, telling her while he can't say how the guy will react, if it goes badly it's not her fault.

Available on: Playstation 3, Playstation 4, Windows, Xbox One

The protagonist NiGHTS is genderless according to the game's director Takashi Iizuka, who says their gender and pronouns are up for interpretation by each individual player, as everyone has different dreams. (Though NiGHTS is still usually referred to with he/him in the games' canon)

Available on: Steam, Sega Saturn, Playstation 2 (exclusive to Japan), Playstation 3, Xbox 360, PC

See Granblue Fantasy (2014).

Available on: Steam, Playstation 4

Justicia reveals that she's a trans woman during her safehouse drop off mission.

Available on: Steam, Playstation 5, Xbox Series X/S

Mizuki is heavily implied to be nonbinary/transfeminine. Their gender is labelled as "?" on the official website for the game. They were male-presenting in middle school, wearing the male school uniform and having shorter hair. In-game, some characters such as An and Miku refer to Mizuki with "they/them" pronouns, although the English translation avoids using pronouns at all. In the Japanese version, the pronoun "boku" is used, which typically refers to young boys casually.

Available on: iOS, Android

From Wikipedia:
Poison is introduced in Final Fight (1989), where she is either a pre-op or post-op trans woman, depending on the region where the game was released.

She is an antagonistic NPC in the arcade beat 'em up games Final Fight (1989) and Mighty Final Fight (1993). She is a playable character in the fighting games Final Fight Revenge (1999), Street Fighter X Tekken (2012), Ultra Street Fighter IV (2014) & Street Fighter V: Arcade Edition (2018).

In Street Fighter V: Arcade Edition, her identity is alluded to more often. Certain dialogue during a conversation with Lucia alludes to the fact that she was born with a gender not matching with her inner being. One of Poison's win quotes, "Being gossiped about is proof of my popularity!" seemingly alludes to the controversy and ambiguity surrounding her gender identity.

Available on: Arcade, Capcom Power System Changer, Amiga, Amstrad CPC, Atari ST, Commodore 64, PlayStation 2, Mega CD, ZX Spectrum, SNES, Sharp X68000, Xbox, XBLA, Game Boy Advance, PlayStation Portable, PlayStation Network, Steam

Mangle, the Puppet, and Funtime Foxy make an appearance in this game.

Available on: PlayStation 4, PlayStation 5, Windows, Nintendo Switch, Oculus Quest, Android, iOS, Xbox One

See Steins;Gate (2009).

Available on: Steam, PlayStation 3, PlayStation 4, PlayStation Vita, Xbox One, Nintendo Switch

At some point after the quest "The Light Within" (released in 2015), the player can show Angof a wedding ring, prompting her to tell the player she was born male, but "corrected" herself to female once she could shapeshift.

There is an entry in her diary that also reads:
"In many ways I am more myself than I have ever been. I am no longer that little boy that felt trapped in a skin that never really was his own. The form changing ability of the crystal certainly has it’s advantages."

Available on: Browser, Steam, Android, iOS